The ProteaPrep Non-ionic Cell Lysis Kit, Mass Spec Grade has been specially designed for the efficient recovery of purified protein lysates from biological samples. The ProteaPrep Lysis Buffer is a proprietary formulation containing a mixture of salts and non-denaturing detergents that has been optimized for efficient solubilization, extraction, and recovery of most proteins during cell lysis. Because the ProteaPrep Non-ionic Cell Lysis Kit is free of lysozyme and harsh detergents (e.g. SDS), the simple protocol permits recovery of a cell lysate that is free from artifactual protein contaminants and that contains soluble, native proteins for downstream characterization and biological testing.
The ProteaPrep Non-ionic Cell Lysis Kit features the Progenta Non-ionic Acid Labile Surfactant I (NALS I), a powerful acid cleavable detergent that alleviates the problems commonly associated with SDS and other detergents in proteomics studies. After completing the cell lysis, the cell extract solution will not interfere with downstream sample preparation and analysis by techniques such as mass spectrometry, enzymatic digestions, 2D gel electrophoresis, and protein quantitation (e.g. Bradford and Lowry assays).