The ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade has been specially designed for the efficient recovery of purified protein lysates from biological samples. The ProteaPrep Anionic Cell Lysis Kit is a proprietary formulation containing a mixture of salts, glycerol, and an acid labile surfactant that has been optimized for efficient solubilization, extraction, and recovery of proteins during cell lysis. Because the ProteaPrep Anionic Cell Lysis Kit is free of lysozyme and harsh detergents (e.g. SDS), the product permits recovery of a cell lysate that is free from artifactual protein contaminants and promotes efficient sample protein solubilization.
The ProteaPrep Anionic Cell Lysis Kit features the Progenta Anionic Acid Labile Surfactant II (AALS II), a powerful acid cleavable detergent that alleviates the problems commonly associated with SDS and other detergents in proteomics studies. After completing the cell lysis, the cell extract solution can be adjusted to a pH of 2.5 - 3 (by TFA, formic acid, etc.) and incubated for 10-20 minutes to fully cleave the AALS into small organic molecules that do not exhibit surfactant activity or interfere with downstream sample preparation and analysis by techniques such as mass spectrometry, enzymatic digestions, 2D gel electrophoresis, and protein quantitation (e.g. Bradford and Lowry assays).