The C4 SpinTips Sample Prep Kit contains tips and reagents for reversed phase solid phase extraction of medium and large protein samples for electrospray or MALDI mass spectrometry. The kit is effective in concentrating and desalting small and large proteins resulting in mass spectra with better S/N and reduced salt adducts.
The SP-152-24 kit contains 24 C4 SpinTips for 20-200 µL volumes, 25 mL RP Reconstitution and Rinse Solution, 25 mL RP Equilibration Solution, 25 mL RP Elution Solution, and 8 SpinTip Adaptors.
The SP-152-96 kit contains 96 C4 SpinTips for 20-200 µL volumes, 50 mL RP Reconstitution and Rinse Solution, 50 mL RP Equilibration Solution, 50 mL RP Elution Solution, and 8 SpinTip Adaptors.
A sample of bovine serum albumin (BSA) was denatured and processed with a C4 SpinTip according to Protocol 1. A total of 500 pmol of the sample was processed and recovered in 50 µL of elution solution, and 1 µL of this solution was directly spotted on a MALDI plate pre-spotted with CHCA matrix (cat# CMS-100).