Glu-C Digestion Kit


 
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Endoproteinase Glu-C, also known an V8, is a serine endoproteinase that is isolated from Staphylococcus aureus V8. Endoproteinase Glu-C is highly purified, resulting in a product suitable for proteomics work.
Our Price: $149.34

Product Code: PE-152-5KIT
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Description

Specification

Research Applications

 
Endoproteinase Glu-C, also known an V8, is a serine endoproteinase that is isolated from Staphylococcus aureus V8. Endoproteinase Glu-C is highly purified, resulting in a product suitable for proteomics work.
The specificity of Glu-C is primarily determined by the buffer pH and composition. In ammonium bicarbonate buffer (pH 7.8), Glu-C preferentially cleaves glutamyl bonds. In phosphate buffers (pH 7.8), Glu-C will cleave at both glutamyl and aspartyl bonds.
However, the presence of a proline residue on the carboxy side of the bond inhibits cleavage. Glu-C is used in proteomics for peptide mapping and protein sequence work due to its highly specific cleavage of peptides resulting in a limited number of fragments.

Data Sets
GLU-C Protease is manufactured using highest quality reagents and tested for protease activity before and after lyophilization with ACTH (1-14) peptide as a substrate. Additionally, samples were verified for MS compatibility.
Features
  • Certified mass Spec Grade enzyme for proteomics applications
  • Quality Assurance tested for specificity and performance
  • Contains all of the necessary reagents for reduction, alkylation, and digestion of protein samples

Quantity Discount

Glu-C, Certified MS Grade
Our Price: $84.00

 

 

 

 

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