The Ultrapure 3-Hydroxypicolinic Acid (3-HPA) is a matrix for use in the analysis of oligonucleotides by matrix assisted laser desorption ionization (MALDI) mass spectrometry. The matrix is recrystallized for maximizing S/N and minimizing salt adducts.
3-Hydroxypicolinic Acid (3-HPA)
Alternative names: 3-Hydroxypyridine-2-carboxylic acid
Molecular weight: 139.11
Molecular formula: C6H5NO3
CAS no.: 874-24-8
MALDI-TOF-MS spectrum of standard oligonucleotides using 3-HPA matrix in dibasic ammonium citrate, acquired in the positive ion mode of an ABI 4800 TOf/TOF instrument. An aliquot of 5 pmol of each oligonucleotide was directly onto a MALDI spot pre-spotted with matrix.