The Progenta Cationic Acid Labile Surfactants are fully mass spec compatible and alleviate the problems commonly associated with other detergents in proteomics studies. At neutral pH, the Progenta CALS functions as a powerful detergent for use in sample preparation, protein solubilization, and cell lysis protocols. After completing the experimental work, the solution is adjusted to a pH of 2.0 with trifluoroacetic acid (TFA) and incubated for 30 minutes at a temperature of 55°C to cleave the CALS into small organic molecules that do not exhibit surfactant activity or interfere with analysis by mass spectrometry. Other commercially available acid labile surfactants require incubation in harsher acidic environments of pH 1.0 to 2.0 for up to several hours and may produce an oily pellet or film from the cleavage by-products. Progenta CALS has been specially engineered to provide powerful surfactant performance with the added benefit of quick, simple acid-based detergent cleavage and removal for reproducible results in mass spectrometry-based proteomics studies.
Both Progenta Cationic Acid Labile Surfactants are effective at solubilizing peptides and proteins, functioning in cell lysis protocols, optimizing enzymatic digestions, and reducing surface adsorption losses via non-specific interactions. CALS I has a Critical Micelle Concentration (CMC) of 3.5 mM. CALS II has a shorter hydrophobic tail, yielding a less powerful CMC of 12.8 mM. Both CALS I and II exhibit fast, efficient degradation kinetics at acidic pH 2.0.